Overexpression of polyphosphate kinase gene (ppk) increases bioinsecticide production by Bacillus thuringiensis

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dc.contributor.authorDoruk, Tugrul
dc.contributor.authorAvican, Ummehan
dc.contributor.authorCamci, Irem Yalim
dc.contributor.authorGedik, Sedef Tunca
dc.date.accessioned2025-10-29T11:24:24Z
dc.date.issued2013
dc.departmentGebze Teknik Üniversitesi
dc.description.abstractPolyphosphate (polyP), synthesized by polyp kinase (PPK) using the terminal phosphate of ATP as substrate, performs important functions in every living cell. The present work reports on the relationship between polyp metabolism and bioinsecticide production in Bacillus thuringiensis subsp. israelensis (Bti). The ppk gene of Bti was cloned into vector pHT315 and the effect of its overexpression on endotoxin production was determined. Endotoxin production by the recombinant strain was found to be consistently higher than that by the wild type strain and the strain that carried the empty plasmid. The toxicity of the recombinant mutant strain (LC50 5.8 +/- 0.6 ng ml(-1)) against late 2nd instar Culex quinquefasciatus was about 7.7 times higher than that of Bti (LC50 44.9 +/- 7 ng ml(-1)). To our knowledge this is the first reported study which relates polyp metabolism with bioinsecticide biosynthesis. (C) 2012 Elsevier GmbH. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TBAG(107T812)]
dc.description.sponsorshipThis work was supported by The Scientific and Technological Research Council of Turkey grant [TBAG(107T812)]. We are thankful to Jacqueline Piret, Melek Ozkan and Carlos Garcia Estrada for critical reading of the manuscript. We are greateful to Oner Kocak for his support in bioassay experiments.
dc.identifier.doi10.1016/j.micres.2012.11.009
dc.identifier.endpage203
dc.identifier.issn0944-5013
dc.identifier.issue4
dc.identifier.orcid0000-0002-0057-320X
dc.identifier.orcid0000-0001-6896-4904
dc.identifier.pmid23369305
dc.identifier.scopus2-s2.0-84875378291
dc.identifier.scopusqualityQ1
dc.identifier.startpage199
dc.identifier.urihttps://doi.org/10.1016/j.micres.2012.11.009
dc.identifier.urihttps://hdl.handle.net/20.500.14854/9923
dc.identifier.volume168
dc.identifier.wosWOS:000317709000003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relation.ispartofMicrobiological Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20251020
dc.subjectPolyphosphate kinase
dc.subjectppk
dc.subjectBacillus thuringiensis
dc.subjectEndotoxin
dc.subjectBioinsecticide
dc.titleOverexpression of polyphosphate kinase gene (ppk) increases bioinsecticide production by Bacillus thuringiensis
dc.typeArticle

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