Arylphosphonate-Tethered Porphyrins: Fluorescence Silencing Speaks a Metal Language in Living Enterocytes**

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dc.contributor.authorKeil, Claudia
dc.contributor.authorKlein, Julia
dc.contributor.authorSchmitt, Franz-Josef
dc.contributor.authorZorlu, Yunus
dc.contributor.authorHaase, Hajo
dc.contributor.authorYuecesan, Guendog
dc.date.accessioned2025-10-29T11:34:26Z
dc.date.issued2021
dc.departmentFakülteler, Temel Bilimler Fakültesi, Kimya Bölümü
dc.description.abstractWe report the application of a highly versatile and engineerable novel sensor platform to monitor biologically significant and toxic metal ions in live human Caco-2 enterocytes. The extended conjugation between the fluorescent porphyrin core and metal ions through aromatic phenylphosphonic acid tethers generates a unique turn off and turn on fluorescence and, in addition, shifts in absorption and emission spectra for zinc, cobalt, cadmium and mercury. The reported fluorescent probes p-H(8)TPPA and m-H(8)TPPA can monitor a wide range of metal ion concentrations via fluorescence titration and also via fluorescence decay curves. Cu- and Zn-induced turn off fluorescence can be differentially reversed by the addition of common chelators. Both p-H(8)TPPA and m-H(8)TPPA readily pass the mammalian cellular membrane due to their amphipathic character as confirmed by confocal microscopic imaging of living enterocytes.
dc.description.sponsorshipDFG
dc.description.sponsorshipG.Y and H.H. would like to thank DFG for funding their work and we would like to thank Dipl.-Ing. Lars Barthel (TU Berlin/Department of Applied and Molecular Microbiology; Prof. Dr. Vera Meyer) for the kind support with the laser scanning confocal microscope. Open access funding enabled and organized by Projekt DEAL.
dc.identifier.doi10.1002/cbic.202100031
dc.identifier.endpage1931
dc.identifier.issn1439-4227
dc.identifier.issn1439-7633
dc.identifier.issue11
dc.identifier.orcid0000-0003-0317-0905
dc.identifier.orcid0000-0002-5105-7280
dc.identifier.pmid33554446
dc.identifier.scopus2-s2.0-85102647560
dc.identifier.scopusqualityQ2
dc.identifier.startpage1925
dc.identifier.urihttps://doi.org/10.1002/cbic.202100031
dc.identifier.urihttps://hdl.handle.net/20.500.14854/12869
dc.identifier.volume22
dc.identifier.wosWOS:000630032800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbh
dc.relation.ispartofChembiochem
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20251020
dc.subjectCaco-2
dc.subjectfluorescence sensors
dc.subjecthomeostasis
dc.subjectmetals
dc.subjecttime-resolved fluorescence
dc.titleArylphosphonate-Tethered Porphyrins: Fluorescence Silencing Speaks a Metal Language in Living Enterocytes**
dc.typeArticle

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