Investigation of Contagious Type Antibiotic Resistance Properties Related with R Plasmids in Escherichia coli Strains Isolated from Izmit Gulfs (Turkey)

Abstract

In this study, 8 Escherichia coli strains one of the pollutions of the Gulf were from the twenty-three seawater samples taken from 9 chosen sites in the Gulf of Izmit, and 8 E. coli coliforms which is one of the pollutions of the Gulf, have been studied with respect to their resistance to antibiotics and their carrier properties of Contagious Types Plasmids with R factor. E. coli samples were isolated and identified by use of classic methods. Sensitivity tests for antibiotics were conducted according to the Kirby-Bauer Disc Diffussion method. The results of E. coli isolates to various antibiotics were as follows; 50% resistance to Tetracyline, 62.5% for Sulbactam/Amphicillin, 62.5% for Penicillin, 50% for Gentamicin, % 12.5 for Amikacin, 37.5% for Chloramphenicol, 25% for Cefoperozone, 37.5% for Kanamycin, and 62.5% for Trimethoprim + Sulphamethoxozale. This study demonstrates the importance of water pollution in the Gulf of Izmit by these pollution indicators, the presence of coliforms. Furthermore, E. coli a water related bacterium, has been proven to be highly resistant to at least 2 antibiotics and to be able to transfer this resistance to R plasmids. In coliform bacteria, presence of high resistance provider R plasmids have been found. This study may effectively help to understandthe resistance models of the E. coli found at the Gulf of Izmit/Turkey.