The first synthesis and characterization of spiro and ansa-cyclotriphosphazenes with 3-Hydroxyflavone and induction of apoptosis in breast cancer (MCF-7)

Abstract

In this study, the first series of 3-Hydroxyflavone substituted spiro, ansa-1,3-propanedioxy and 2,2-dimethyl 1,3propanedioxy substituted cyclotriphosphazene compounds 912 were synthesized and their structures were characterized by MALDI-TOF MS, NMR (31P and 1H) and FT-IR spectroscopies. After the antioxidant (DPPH), total phenolic, and cytotoxicity (WST-8) activities of the compounds were determined, the mRNA expressions of multidrug resistance and apoptosis effects were investigated by RT-PCR analysis of the compounds in MCF-7 cells. While the DPPH activity of the compounds is 90.56%, the total phenolic activity is 187.01 mg. Cytotoxic activity IC50: 23.07 mu M was detected in breast cell line. In addition, the cell death gene expression values of the compounds showed an increase in BAX, p53, Caspase-3 and Caspase-7 gene expressions, and a decrease in BCL-2. The compounds were determined to be notable for their effective cytotoxicity data in breast cancer (MCF7) and indicators of apoptosis through BAX regulation.