Screening of CCDC43 molecular partners by BioID2-based proximity labeling

Abstract

Background/aim: CCDC43 (coiled-coil domain containing protein 43) is a eukaryotic protein that contains alpha-helical domains in its structure, consistent with the family to which it belongs. It is predominantly located in the cytosol of the cell. CCDC43 protein has been shown to play a role in cell proliferation, invasion, metastasis, and epithelial-mesenchymal transition in gastric and colorectal cancer types. The fundamental function and cellular interaction network of this protein, which is known to have varying expression levels in various cancer types, has not yet been fully elucidated. Here, we conducted a proximity-dependent biotin identification (BioID2) screening approach for CCDC43 to uncover proximity interactors. Materials and methods: Using mass spectrometry of streptavidin pull-down of biotinylated proteins with filtering approaches, we identified candidate protein interactors for CCDC43. Results: We suggest an association between CCDC43 and RNA-binding proteins based on all our biological replicates. The strongest candidate for the interactome is YBX1, a highly conserved cold shock domain and nucleic acid-binding protein that has multiple essential functions in the cell. Conclusion: These findings suggest that CCDC43 may play a role in critical pathways within the cell.