Synthesis and characterization of novel multifunctional SiO2 nanoparticles

Abstract

In this study, the synthesis and characterization of multifunctionalized silica nanoparticles for biomedical applications were clarified. For the preparation of azole functional Si0<inf>2</inf>, a two-step method based on hydrolyzed of TEOS was used in this study. The epoxy functional Si0<inf>2</inf> nanoparticles modified with 5-Amino-Tetrazole (ATet-Si0<inf>2</inf>), in order to obtain 1,2,4-5-Amino-Tetrazole functional Si02 molecules via ring opening of the epoxy ring. FT-IR, SEM, TEM and AFM analysis were confirmed the functionalization of Si0<inf>2</inf>. TGA and DSC were employed to examine the thermal stability and homogeneity of the materials. The thermal and proton conducting property of material was investigated under anhydrous state. TGA showed that the samples are thermally stable up to approximately 250°C. anticancer effects of Aminotetrazole was investigated at concentrations (10, 50, 100, 500, 1000, 5000ug/ml) on Hela cells during 72h exposure using the xCELLigence system. Our data suggests that the xCELLigence live cell analysis system offers dynamic to use ATet-Si02, as an alternative anticancer drug. The xCELLigence system can be used as a rapid monitoring tool for cellular viability and be applied in anticancer testing of chemical substances using in vitro cell cultures. © 2017 Elsevier B.V., All rights reserved.