Liquid crystalline phthalocyanine thin films as nanoscale substrates for protein adsorption

Abstract

Bovine Serum Albumin (BSA) protein was adsorbed on nanoscale thick spin cast film of liquid crystalline copper octakishexylthiophthalocyanine [(C<inf>6</inf>S)<inf>8</inf>PcCu]. The effect of heat treatment of films on BSA adsorption was investigated using ellipsometry, scanning electron microscopy and UV-Visible absorption spectroscopy. The ordered layer after heat treatment demonstrated quite different adsorption pattern. Heat treatment produced changes in the orientation of phthalocyanine stacks, making favourable contribution to the protein adsorption and surface coverage profile. Finally epitope presentation on bound BSAs was examined by using anti-BSA antibody. Copyright © 2008 American Scientific Publishers All rights reserved. © 2009 Elsevier B.V., All rights reserved.