Effect of Scaling up on Bioetanol Production from Sugar Beet Molasses by Vitreoscilla Hemoglobin Expressing Escherichia coli Strains

Abstract

In the present work, bioethanol production through VHb expressing Escherichia coli TS3 and TS4 strains from sugar-beet molasses hydrolysate containing media was investigated. The highest growth and ethanol production were obtained in MB2 (contains about 4% sugar) medium. In MB2 medium, bioethanol production was enhanced by E. coli TS3 and TS4 strains from small scale to big scale fermentation. The highest ethanol productions by TS3 and TS4 strains were determined as 2.49% and 2.62% (v/v) respectively, with the largest volume of fermentation medium. It was shown that scaling up process had positive effect on bioethanol production from sugar beet molasses through VHb expressing strains. © 2023 Elsevier B.V., All rights reserved.