Preparation of isocyanate-containing hydrogel films as antibacterial enzyme immobilization matrices

Abstract

Sulfobetaine-based isocyanate-containing hydrogel films were synthesized on glass surfaces and the immobilization of small molecules and proteins onto those films was demonstrated. Synthesis of hydrogel films and their functionalizations were confirmed using FTIR spectroscopy. Obtained polymer films were used as matrices for the immobilization of the antibacterial enzyme lysostaphin (Lys). Successful immobilization of Lys enzyme was demonstrated using FTIR and Bradford analyses. The antibacterial activity of enzyme-immobilized surfaces against different Staphylococcus aureus (S. aureus) bacterial strains was evaluated. Enzyme-conjugated surfaces showed 90% killing efficacy against S. aureus 35 bacteria, and the antibacterial activity of those surfaces was maintained for at least 30 days and multiple exposures to the bacteria. The enzyme-immobilized hydrogel coatings reported here provide a promising antibacterial surface against S. aureus.