Affinity tag effect on the salt rejection potential of Halomonas elongata aquaporin incorporated in thin film nanocomposite membrane

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dc.contributor.authorYilmaz, Hilal
dc.contributor.authorOzdemir, Fatma Inci
dc.contributor.authorErgenekon, Pınar
dc.contributor.authorÖzkan, Melek
dc.date.accessioned2025-10-29T11:23:53Z
dc.date.issued2020
dc.departmentFakülteler, Mühendislik Fakültesi, Çevre Mühendisliği Bölümü
dc.description.abstractIn this study, effect of affinity tags, Histidine (His) and Glutathione-S-Transferase (GST), on the activity of halophilic aquaporin was analyzed. The gene coding for H. elongata aquaporin was cloned into pET28a vector and expressed in E. coli. BL21 successfully. Stopped flow light scattering measurements showed that His-tagged aquaporin is functional. The difference in the filtration parameters caused by affinity tags were determined by using thin film composite nano-filtration (NFC) membranes prepared with the aquaporins. At 100 mM salt concentration, water permeability (L/m(2).h) and the % salt rejection of NFC membranes produced with the His-tagged aquaporin was found to be higher than that of the membrane with GST-tagged aquaporin. Salt rejection of His-tagged aquaporin-membrane was found to be 53% with a lower solute permeability value (B). Use of short affinity tag (His tag) for cloning resulted in higher solute rejection ability of TFC membranes prepared with H. elongata aquaporins.
dc.description.sponsorshipScientific and Technological Research Council of Turkey TUBITAK [:115Z245]
dc.description.sponsorshipGebze Technical University [GTU BAP 2018 A101-10]
dc.description.sponsorshipWe thank The Scientific and Technological Research Council of Turkey TUBITAK (Project No:115Z245) and Gebze Technical University (Project No: GTU BAP 2018 A101-10) for supporting this study. We also thank Assoc. Prof. Dr. Ferruh OZCAN for his help in Western blotting experiments.
dc.identifier.doi10.1016/j.pep.2020.105664
dc.identifier.issn1046-5928
dc.identifier.issn1096-0279
dc.identifier.orcid0000-0003-0818-103X
dc.identifier.orcid0000-0002-5782-0283
dc.identifier.orcid0000-0001-9017-5389
dc.identifier.pmid32380098
dc.identifier.scopus2-s2.0-85084405176
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1016/j.pep.2020.105664
dc.identifier.urihttps://hdl.handle.net/20.500.14854/9659
dc.identifier.volume173
dc.identifier.wosWOS:000534264900004
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAcademic Press Inc Elsevier Science
dc.relation.ispartofProtein Expression and Purification
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20251020
dc.subjectAffinity tags
dc.subjectAquaporins
dc.subjectBiomimetic membranes
dc.subjectDesalination
dc.subjectHalomonas elongata
dc.titleAffinity tag effect on the salt rejection potential of Halomonas elongata aquaporin incorporated in thin film nanocomposite membrane
dc.typeArticle

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