Application of mixed self-assembled monolayers (Mixed SAMs) for nucleic acid detection

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dc.contributor.authorOral, Zehra Banu Bahsi
dc.date.accessioned2025-10-29T11:24:31Z
dc.date.issued2016
dc.departmentGebze Teknik Üniversitesi
dc.description.abstractMixed self-assembled monolayers (Mixed SAMs) consisting of 8-Ferrocenyl-1-octanethiol and 6-Mercapto1-hexanol (FcOT:MCH) with probe PNA on gold electrodes were fabricated by using two-step after the optimization of immobilization temperature of FcOT:MCH SAMs. Using AC voltammetry, a novel nucleic acid detection platform, with mixed SAMs, was proposed. A negative formal potential shift was observed after complementary ssDNA hybridization while there was no significant difference after non complementary ssDNA hybridization. Compatible results were obtained with the measurement of formal potential differences between mixed SAMs and target DNA (complementary and non-complementary DNA) in different target DNA concentrations. The formal potential difference between mixed SAMs immobilization and complementary ssDNA hybridization was measured in different ionic strength concentrations. (C) 2016 Elsevier Ltd. All rights reserved.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) at University of Cambridge
dc.description.sponsorshipZ.B. Bahsi Oral acknowledges funded from The Scientific and Technological Research Council of Turkey (TUBITAK) as a Post Doctoral training at University of Cambridge.
dc.identifier.doi10.1016/j.measurement.2016.07.060
dc.identifier.endpage4
dc.identifier.issn0263-2241
dc.identifier.issn1873-412X
dc.identifier.orcid0000-0001-5513-0847
dc.identifier.startpage1
dc.identifier.urihttps://doi.org/10.1016/j.measurement.2016.07.060
dc.identifier.urihttps://hdl.handle.net/20.500.14854/9963
dc.identifier.volume94
dc.identifier.wosWOS:000390512100001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.institutionauthorOral, Zehra Banu Bahsi
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.relation.ispartofMeasurement
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20251020
dc.subjectAlkanethiol Peptide Nucleic Acid (PNA)
dc.subjectAC voltammetry
dc.subjectAmperometric biosensors
dc.titleApplication of mixed self-assembled monolayers (Mixed SAMs) for nucleic acid detection
dc.typeArticle

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