Evaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP)

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dc.contributor.authorCaydasi, Ayse Koca
dc.contributor.authorPereira, Gislene
dc.coverage.doi10.1007/978-1-4939-6502-1
dc.date.accessioned2025-10-29T11:33:35Z
dc.date.issued2017
dc.departmentGebze Teknik Üniversitesi
dc.description.abstractFluorescence recovery after photobleaching (FRAP) is a powerful technique to study in vivo binding and diffusion dynamics of fluorescently labeled proteins. In this chapter, we describe how to determine spindle pole body (SPB) binding dynamics of mitotic exit network (MEN) and spindle position checkpoint (SPOC) proteins using FRAP microscopy. Procedures presented here include the growth of the yeast cultures, sample preparation, image acquisition and analysis.
dc.identifier.doi10.1007/978-1-4939-6502-1_13
dc.identifier.endpage182
dc.identifier.isbn978-1-4939-6502-1
dc.identifier.isbn978-1-4939-6500-7
dc.identifier.issn1064-3745
dc.identifier.orcid0000-0003-2570-1367
dc.identifier.pmid27826864
dc.identifier.scopus2-s2.0-84994895745
dc.identifier.scopusqualityQ4
dc.identifier.startpage167
dc.identifier.urihttps://doi.org/10.1007/978-1-4939-6502-1_13
dc.identifier.urihttps://hdl.handle.net/20.500.14854/12486
dc.identifier.volume1505
dc.identifier.wosWOS:000399603100014
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc
dc.relation.ispartofMitotic Exit Network: Methods and Protocols
dc.relation.publicationcategoryKitap Bölümü - Uluslararası
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20251020
dc.subjectFRAP
dc.subjectBinding dynamics
dc.subjectCentrosome
dc.subjectSpindle pole body
dc.subjectSPB
dc.subjectMEN
dc.subjectSPOC
dc.subjectSpindle position checkpoint
dc.titleEvaluation of the Dynamicity of Mitotic Exit Network and Spindle Position Checkpoint Components on Spindle Pole Bodies by Fluorescence Recovery After Photobleaching (FRAP)
dc.typeBook Chapter

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