Synthesis and characterization of new cyclotriphosphazene compounds

Abstract

In the present study, Spiro (1a), dispiro (1b, 2, 3), per-substituted spermine-bridged (6-9) and dispiroansa spermine (10) derivatives of cyclotriphosphazene have been synthesized. The structures of the novel compounds (1b, 6-10) have been characterized by elemental analysis, FTIR, mass spectrometry, H-1 and P-31 NMR spectroscopy. The molecular structures of 1b, 2, 8, and 10 were determined by single crystal X-ray crystallography. In order to investigate the anti-tumour properties of the newly synthesized cyclotriphosphazene derivatives, in vitro cytotoxic activity test (MTT assay) has been performed using HT-29 (human colon adenocarcinoma) and Hep2 (human epidermoid larynx carcinoma) cell lines. The result of the MU assay showed that while compound 1a has cytotoxic effect on both Hep2 and HT-29 cell lines, compound 3 has only cytotoxic effect towards the Hep 2 cells. (C) 2012 Elsevier Ltd. All rights reserved.