Author Correction: Effect of iron and magnesium addition on population dynamics and high value product of microalgae grown in anaerobic liquid digestate (Scientific Reports, (2020), 10, 1, (3510), 10.1038/s41598-020-60622-1)

Abstract

Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515 f (F), 5?-GTG CCA GCMGCC GCG GTAA-3? and 806r (R), 5?-GGA CTA CHVHHHTWT CTA AT-3?] for 16S, TAReuk454FWD1 (F), 5?-CCA GCA SCYG CGG TAA TTC -3? and TAReukREV3 (R), 5?-ACT TTC GTT CTT GAT YRA -3? primers for 18S rDNA, p23SrV_f1 (F), 5?-GGA CAG AAA GAC CCT ATG AA-3? and p23SrV_r1 (R), 5?-TCA GCC TGT-TAT CCC TAGAG-3? primers for 23S rDNA, (F) 5?-TGA AAC AGAAMAWC GTC ATT-3? and (R) 5?-CCTTCNCGAATMGCR AAW -3? primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” should read: “PCR amplification and sequence analyses of 16S rRNA, 18S rRNA, 23S rRNA and tufa Molecular confirmation of isolates was performed via next generation sequencing of 16S/18S/23S rRNA and tufA marker regions. Genomic DNA from different mixed microalgae culture samples was isolated and high-throughput sequencing analysis was applied to each sample. Targeted amplicon libraries were constructed with universal V4 region primers [515f (F), 5?-GTG YCA GCMGCC GCG GTAA-3?1 and 806r (R), 5?-GGA CTA CNVGGG TWT CTAAT-3?2] for 16S, TAReuk454FWD1 (F), 5?-CCA GCA SCYG CGG TAA TTC -3? and TAReukREV3 (R), 5?-ACT TTC GTT CTT GAT YRA -3? primers3 for 18S rDNA, p23SrV_f1 (F), 5?-GGA CAG AAA GAC CCT ATG AA-3? and p23SrV_r1 (R), 5?-TCA GCC TGT TAT CCC TAG AG-3? primers4 for 23S rDNA, (F) 5?-TGA AAC AGAAMAWC GTC ATT-3? and (R) 5?-CCTTCNCGAATMGCR AAW -3? primers for elongation factor tufA. Purified-amplicon libraries were sequenced using an Illumina MiSeq platform (2 × 300 paired-end reads).” The omitted References are listed below as References 1–4 respectively. © 2020 Elsevier B.V., All rights reserved.