Quantification of Secondary Metabolites of Satureja pilosa (Lamiaceae) by LC-HRMS and Evaluation of Antioxidant and Cholinergic Activities

Abstract

In this study, secondary metabolite profiles of dichloromethane, acetone and methanol extracts of leaves and branches of Satureja pilosa species were determined by LC-HRMS. In-vitro inhibition activities of the obtained extracts against Alzheimer's disease (AD) which is a neurodegenerative disease were performed, and structure-activity relationships were discussed. While rosmarinic acid was found to be at the highest rate among the reported secondary metabolites in acetone and methanol extracts of the plant, penduletin was the secondary metabolite with the highest concentration in dichloromethane extract of the plant. The highest AChE inhibition was determined in leaf methanol extract with IC50: 41.2 +/- 5.6 mu g/mL, and the highest BChE inhibition was detected in leaf dichloromethane extract of the species with IC50: 52.3 +/- 8.6 mu g/mL concentration. Herein, total antioxidant capacities of the extracts were determined and reported by DPPH and ABTS radical scavenging and CUPRAC reducing ability methods.