A Novel Biosensor Based on Laccase for The Detection of Catechol

Abstract

Phenolic compounds are broadly used in petrol industrial production processes and thus they arehazardous for ground and surface water and environment. Many of them have harmful effects onplants, animals and human health. Laccases can oxide phenol and phenolic compounds. Biosensorsprovide ideal sensing systems to determine phenolic compounds. In the present paper, it wasdeveloped novel sensor surface with modified laccase on gold electrode which could recognize thephenolic compound catechol. The electrode arrays integrated microfluidics have been fabricated andcharacterized in order to create a sensor chip which is easy, rapid, and cheaper to produce also havegood electrochemical sensing properties. This gold electrode was used to obtain laccase activesurface. Laccase was covalently bounded to self-assembled monolayer on gold electrode viaglutaraldehyde reactions. Cyclic voltammetry and amperometry activity assays showed that thedetection of catechol with laccase modified gold surface. All of the study was carried out usingautomated biosensor device. Linear range for this biosensor was 0.025 mM to 0.8 mM.