A NEW POTENTIOMETRIC SENSOR FOR THE ASSAY OF P53 IN BLOOD SAMPLES

Abstract

A potentiometric sensor based on graphite paste modified with a phthalocyanine-BODIPY conjugate was proposed for the assay of tumor suppressor P53. The working concentration range was between 1.17x10(-9) and 2.93x10(-8)x g/mu L with a slope of 82.62mV/mu g mL(-1) and a limit of detection was 3.37x10(-10)xg/mu L. The selectivity of the sensor was checked against possible interfering species from blood samples. The sensor was applied for the analysis of P53 in whole blood samples, without any pretreatment of the sample.